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pires centrin1 9 mcherry  (Addgene inc)


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    Structured Review

    Addgene inc pires centrin1 9 mcherry
    Pires Centrin1 9 Mcherry, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pires+centrin1+9+mcherry/pIRES+Centrin1+mCherry+(Plasmid+%2364338)/pm31042281-186-31-39
    Average 91 stars, based on 3 article reviews
    pires centrin1 9 mcherry - by Bioz Stars, 2026-09
    91/100 stars

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    other:

    Article Title: Human IFT52 mutations uncover a novel role for the protein in microtubule dynamics and centrosome cohesion.
    Article Snippet: 10 For zebrafish rescue experiments, full length zebrafish ift52 coding sequence was amplified by 11 RT-PCR from total 24hpf cDNA and cloned into pCS2 plasmid according to the Cold Fusion 12 kit protocol (SBI System Biosciences), using the following primers: zift52-pCS2_F: 5'- 13 acgactcactatagttctagAGCTCCACTGCAGATTGAGT-3' and zift52-pCS2_R: 5'- 14 gctacttgttctttttgcagCGCATTATAACTCCCCACAGC-3'.

    Mutagenesis:

    Article Title: Human IFT52 mutations uncover a novel role for the protein in microtubule dynamics and centrosome cohesion.
    Article Snippet: .. The missense mutations were 7 obtained by site-directed mutagenesis using Pfu Turbo kit (Invitrogen) and deletion of the exon 8 6 was created using Q5 site-directed mutagenesis kit (New England Biolabs). pIRES-Centrin1-9 mCherry was a gift from Matthieu Piel (Addgene plasmid #64340). .. 10 For zebrafish rescue experiments, full length zebrafish ift52 coding sequence was amplified by 11 RT-PCR from total 24hpf cDNA and cloned into pCS2 plasmid according to the Cold Fusion 12 kit protocol (SBI System Biosciences), using the following primers: zift52-pCS2_F: 5'- 13 acgactcactatagttctagAGCTCCACTGCAGATTGAGT-3' and zift52-pCS2_R: 5'- 14 gctacttgttctttttgcagCGCATTATAACTCCCCACAGC-3'.

    Plasmid Preparation:

    Article Title: Human IFT52 mutations uncover a novel role for the protein in microtubule dynamics and centrosome cohesion.
    Article Snippet: .. The missense mutations were 7 obtained by site-directed mutagenesis using Pfu Turbo kit (Invitrogen) and deletion of the exon 8 6 was created using Q5 site-directed mutagenesis kit (New England Biolabs). pIRES-Centrin1-9 mCherry was a gift from Matthieu Piel (Addgene plasmid #64340). .. 10 For zebrafish rescue experiments, full length zebrafish ift52 coding sequence was amplified by 11 RT-PCR from total 24hpf cDNA and cloned into pCS2 plasmid according to the Cold Fusion 12 kit protocol (SBI System Biosciences), using the following primers: zift52-pCS2_F: 5'- 13 acgactcactatagttctagAGCTCCACTGCAGATTGAGT-3' and zift52-pCS2_R: 5'- 14 gctacttgttctttttgcagCGCATTATAACTCCCCACAGC-3'.



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    91
    Addgene inc pires centrin1 9 mcherry
    Pires Centrin1 9 Mcherry, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pires+centrin1+9+mcherry/pIRES+Centrin1+mCherry+(Plasmid+%2364338)/pm31042281-186-31-39
    Average 91 stars, based on 1 article reviews
    pires centrin1 9 mcherry - by Bioz Stars, 2026-09
    91/100 stars
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